Table 4 Quadratic model ANOVA and optimization for protoplast transfection efficiency
From: Optimization of isolation and transfection conditions of maize endosperm protoplasts
Source | Sum of squares | DF | Mean square | F-value | p-value | R-Square |
|---|---|---|---|---|---|---|
Model | 43,683,750 | 9 | 4,853,750 | 41.82,179 | < 0.0001 S | 0.9666 |
X1-Protoplast Conc. | 8,769,727 | 1 | 8,769,727 | 75.56,336 | < 0.0001 | |
X2-Total Plasmid DNA | 22,592.06 | 1 | 22,592.06 | 0.194662 | 0.666,314 | |
X3-Endosperm Age | 55,999.63 | 1 | 55,999.63 | 0.482514 | 0.499,513 | |
X1X2 | 2.88 | 1 | 2.88 | 2.48E−05 | 0.996101 | |
X1X3 | 5703.12 | 1 | 5703.12 | 0.04914 | 0.82801 | |
X2X3 | 208,658 | 1 | 208658 | 1.797878 | 0.202929 | |
X21 | 10,755,781 | 1 | 10,755,781 | 92.67597 | < 0.0001 | |
X22 | 12,379,165 | 1 | 12,379,165 | 106.6637 | < 0.0001 | |
X23 | 20,139,145 | 1 | 20,139,145 | 173.5267 | < 0.0001 | |
Residual | 1,508,753 | 13 | 116,057.9 | |||
Lack of Fit | 713,758.2 | 5 | 142,751.6 | 1.436,504 | 0.0385 NS | |
Pure Error | 794,994.8 | 8 | 99,374.34 | |||
Corrected Total | 45,192,503 | 22 |
Factor | Name | Optimal level | Experimental level range | |||
|---|---|---|---|---|---|---|
Optimum | Lowest | Highest | ||||
Optimization for protoplast transfection efficiency | ||||||
X1 | Protoplast concentration (106/ml) | 1.5 | 0.5 | 2.5 | ||
X2 | Total plasmid DNA (µg) | 10 | 5 | 15 | ||
X3 | Endosperm Age (DAP) | 8 | 6 | 10 | ||
Predicted response | Transfection efficiency (%) | Standard deviation | 95% CI low | 95% CI high | ||
|---|---|---|---|---|---|---|
64.8 | 0.26 | 63 | 68.4 |