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Table 4 Quadratic model ANOVA and optimization for protoplast transfection efficiency

From: Optimization of isolation and transfection conditions of maize endosperm protoplasts

Source Sum of squares DF Mean square F-value p-value R-Square
Model 43,683,750 9 4,853,750 41.82,179 < 0.0001 S 0.9666
X1-Protoplast Conc. 8,769,727 1 8,769,727 75.56,336 < 0.0001  
X2-Total Plasmid DNA 22,592.06 1 22,592.06 0.194662 0.666,314  
X3-Endosperm Age 55,999.63 1 55,999.63 0.482514 0.499,513  
X1X2 2.88 1 2.88 2.48E−05 0.996101  
X1X3 5703.12 1 5703.12 0.04914 0.82801  
X2X3 208,658 1 208658 1.797878 0.202929  
X21 10,755,781 1 10,755,781 92.67597 < 0.0001  
X22 12,379,165 1 12,379,165 106.6637 < 0.0001  
X23 20,139,145 1 20,139,145 173.5267 < 0.0001  
Residual 1,508,753 13 116,057.9    
Lack of Fit 713,758.2 5 142,751.6 1.436,504 0.0385  NS  
Pure Error 794,994.8 8 99,374.34    
Corrected Total 45,192,503 22     
Factor Name Optimal level Experimental level range   
Optimum Lowest Highest   
Optimization for protoplast transfection efficiency
X1 Protoplast concentration (106/ml) 1.5 0.5 2.5   
X2 Total plasmid DNA (µg) 10 5 15   
X3 Endosperm Age (DAP) 8 6 10   
Predicted response Transfection efficiency (%) Standard deviation 95% CI low 95% CI high   
  64.8 0.26 63 68.4   
  1. N Significant, NS non-significant, SE standard error, CI confidence interval