Table 1 Experimental and coded levels used in box-behnken design for studying the effects of cellulase (x1), macerozyme (x2), mannitol (x3) and hydrolysis time (x4) on yield of isolated protoplasts along with the predicted mean and observed responses, and FDA results
From: Optimization of isolation and transfection conditions of maize endosperm protoplasts
Experimental factor | Coded symbol | Coded variable levels | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Low | Centre | High | |||||||||
−1 | 0 | +1 | |||||||||
Cellulase (%) | X1 | 1.00 | 1.50 | 2.00 | |||||||
Macerozyme (%) | X2 | 0.50 | 0.75 | 1.00 | |||||||
Mannitol | X3 | 0.40 | 0.60 | 0.80 | |||||||
Hydrolysis time (h) | X4 | 4 | 6 | 8 | |||||||
Test Number | Coded levels | Actual levels | Response | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Yield, Y (×106 cells/ml) | FDA | ||||||||||
X1 | X2 | X3 | X4 | X1 | X2 | X3 | X4 | Observed Y | Predicted Y | (%) | |
Coded levels for the four factors selected for protoplast isolation | |||||||||||
1 | +1 | +1 | 0 | 0 | 2.00 | 1.00 | 0.60 | 6 | 0.95 | 1.52 | 92.3 |
2 | +1 | −1 | 0 | 0 | 2.00 | 0.50 | 0.60 | 6 | 1.45 | 1.68 | 93.9 |
3 | −1 | +1 | 0 | 0 | 1.00 | 1.00 | 0.60 | 6 | 1.25 | 1.49 | 90.9 |
4 | −1 | −1 | 0 | 0 | 1.00 | 0.50 | 0.60 | 6 | 1.10 | 1.00 | 95.8 |
5 | +1 | 0 | +1 | 0 | 2.00 | 0.75 | 0.80 | 6 | 1.55 | 2.22 | 95.1 |
6 | +1 | 0 | −1 | 0 | 2.00 | 0.75 | 0.40 | 6 | 2.70 | 2.56 | 97.2 |
7 | −1 | 0 | +1 | 0 | 1.00 | 0.75 | 0.80 | 6 | 1.70 | 1.65 | 94.6 |
8 | −1 | 0 | −1 | 0 | 1.00 | 0.75 | 0.40 | 6 | 3.30 | 2.43 | 96.9 |
9 | +1 | 0 | 0 | +1 | 2.00 | 0.75 | 0.60 | 8 | 1.70 | 1.02 | 90.3 |
10 | +1 | 0 | 0 | −1 | 2.00 | 0.75 | 0.60 | 4 | 2.85 | 2.21 | 95.1 |
11 | −1 | 0 | 0 | +1 | 1.00 | 0.75 | 0.60 | 8 | 0.85 | 1.22 | 90.5 |
12 | −1 | 0 | 0 | −1 | 1.00 | 0.75 | 0.60 | 4 | 0.90 | 1.31 | 92.0 |
13 | 0 | +1 | +1 | 0 | 1.50 | 1.00 | 0.80 | 6 | 3.00 | 2.02 | 96.0 |
14 | 0 | + | −1 | 0 | 1.50 | 1.00 | 0.40 | 6 | 1.70 | 1.75 | 91.8 |
15 | 0 | −1 | +1 | 0 | 1.50 | 0.50 | 0.80 | 6 | 1.35 | 1.03 | 94.1 |
16 | 0 | −1 | −1 | 0 | 1.50 | 0.50 | 0.40 | 6 | 1.70 | 2.41 | 91.5 |
17 | 0 | +1 | 0 | +1 | 1.50 | 1.00 | 0.60 | 8 | 0.95 | 0.79 | 96.4 |
18 | 0 | +1 | 0 | −1 | 1.50 | 1.00 | 0.60 | 4 | 1.15 | 1.43 | 92.3 |
19 | 0 | −1 | 0 | +1 | 1.50 | 0.50 | 0.60 | 8 | 1.10 | 0.62 | 91.7 |
20 | 0 | −1 | 0 | −1 | 1.50 | 0.50 | 0.60 | 4 | 1.30 | 1.26 | 93.8 |
21 | 0 | 0 | +1 | +1 | 1.50 | 0.75 | 0.80 | 8 | 1.30 | 1.88 | 95.8 |
22 | 0 | 0 | +1 | −1 | 1.50 | 0.75 | 0.80 | 4 | 1.10 | 1.20 | 97.3 |
23 | 0 | 0 | −1 | +1 | 1.50 | 0.75 | 0.40 | 8 | 0.75 | 1.12 | 93.3 |
24 | 0 | 0 | −1 | −1 | 1.50 | 0.75 | 0.40 | 4 | 3.20 | 3.08 | 93.8 |
25 | 0 | 0 | 0 | 0 | 1.50 | 0.75 | 0.60 | 6 | 7.75 | 8.03 | 93.3 |
26 | 0 | 0 | 0 | 0 | 1.50 | 0.75 | 0.60 | 6 | 8.50 | 8.03 | 93.9 |
27 | 0 | 0 | 0 | 0 | 1.50 | 0.75 | 0.60 | 6 | 7.60 | 8.03 | 91.8 |
28 | 0 | 0 | 0 | 0 | 1.50 | 0.75 | 0.60 | 6 | 8.25 | 8.03 | 96.7 |
29 | 0 | 0 | 0 | 0 | 1.50 | 0.75 | 0.60 | 6 | 8.05 | 8.03 | 94.1 |