Fig. 2

Length distributions and mappability of reads. a–c the length distributions of PacBio subreads (a), ONT Dc 1D reads (b), and ONT Pc 1D reads (c). Aligned reads are colour coded to indicate the fractions of reads that are unaligned (white), trans-chimeric alignments (blue), self-chimeric alignments (purple), gapped alignments consisting of multiple paths (red), and single best alignments (grey).The leftmost bar represents all reads, the middle portion represents reads from 0 to 4 kb in length, and the rightmost represents reads > 4 kb in length. d–f the mappability of different length bins of PacBio subreads (d), ONT Dc 1D reads (e), and ONT Pc 1D reads (f). The leftmost bar represents the fraction of the mappable read length of the total read length for all reads. The middle section shows the mappable fraction for read lengths ranging from 0 to 4 kb in 500-bp increments, and the rightmost bar represents the mappable fraction of reads > 4 kb