Comparison of three Agrobacterium-mediated co-transformation methods for generating marker-free transgenic Brassica napus plants

Table 2 Co-transformation frequencies in T₀ transformants of the mixed-strain system and the “double T-DNA” vector system of pDB1300-3300

Strains transformed	Number of transformants
Strains transformed	Rooted plants	HPT⁺ plants	Bar⁺HPT⁺	Bar⁻HPT⁺	Co-transformation frequency (%)
pCAMBIA1300/3300 (1:8)	58	58	24	34	41
pCAMBIA1300/3300 (1:4)	28	28	17	11	61
pCAMBIA1300/3300 (1:2)	34	34	22	12	65
pCAMBIA1300/3300 (1:1)	57	53	43	10	81
pCAMBIA1300/3300 (2:1)	90	89	38	51	43
pCAMBIA1300/3300 (4:1)	21	21	5	16	24
pCAMBIA1300/3300 (8:1)	91	87	24	63	28
Total	379	370	173	197	47
pDB1300-3300	26	26	17	9	65

ISSN: 1746-4811