Table 2 List of primers used in this study
From: A cell suspension based uptake method to study high affinity glucosinolate transporters
S. no. | Primer | Sequence (5′-3′) |
|---|---|---|
Gene amplification | ||
 1 | At3G47960 GTR1 FP | CACCATGAAGAGCAGAGTCATTCTTAACC |
 2 | At3G47960 GTR1 RP | TCAGACAGAGTTCTTGTCTTGTAGC |
 3 | At5G62680 GTR2 FP | CACCATGGAGAGAAAGCCTCTTGAAC |
 4 | At5G62680 GTR2 RP | TCAGGCAACGTTCTTGTCTTGCTG |
Transgene insertion validation | ||
 5 | Bar FP | GCTCTACACCCACCTGCTGAA-3′ |
 6 | Bar RP | TCAGATCTCGGTGACGG-3′ |
 7 | GTR1 internal FP | ATCAACAGTTTCTTCAACTGGT |
 8 | GTR2 internal FP | TGGGGCTGATCAGTTTAACC |
 9 | GTR1/GTR2 internal RP | ACTGCTTGTAGTAAAACTCCAT |
Transgene expression validation | ||
 10 | AtGTR1(RT) FP | GAATCGGAGCTGGGTTTACA |
 11 | AtGTR2(RT) FP | TGGCATTTTCTTCGCTACCGC |
 12 | GTR1/GTR2 (RT) RP | ACTGCTTGTAGTAAAACTCCAT |
 13 | Ubuiquitin FP | GGTGGGATGCAAATCTTCGTGAAAAC |
 14 | Ubuiquitin RP | CTGGATGTTGTAGTCGGCCAAGGTA |