Fig. 3
From: Ice accommodation in plant tissues pinpointed by cryo-microscopy in reflected-polarised-light
Leaves were clamped between sponge rubber (sr) by tightening the butterfly nuts (bn) (a). Leaves were immersed in a solution of water with ice nucleation active (INA) bacteria to trigger ice nucleation and to avoid artificial supercooling. Sectioning was performed with a razorblade, which was guided during cutting by the plane surface of the sample holder. Leaves were cut one at a time and, thereafter, immediately inspected with CMrpl. b Dimensions in millimetres are shown in the technical drawing; sponge rubber is indicated by the hatched area