Fig. 3

Schematic decision-making flow chart to efficiently carry out the various steps in the RNAscope assay. A rhombus represents the decision-making step, smooth edge rectangles represent a process, a storage symbol depicts the step for long term sample storage and terminator represents the end of a successful RNAscope experimental pipeline. Green bars represent a successful progression of the assay, whereas a red arrow represents a possible failure point that may require returning to the previous step (black arrows). O Optimal, S Suboptimal