Fig. 4
mcs1 and mci1 exhibited abnormal responses to HCO3−/CO2 with respect to [Ca2+]cys changes and stomatal movement. a The mcs1 mutant is hypersensitive to HCO3−/CO2 treatment. (Left) Images of wild-type and mcs1 epidermal strips were taken, and guard cell images before and 30 min after addition of 1 mM KHCO3 are shown. (Right) Changes in the apertures (width:length) of stomatal pores in wild type and mcs1 in response to 1 mM KHCO3. Data from three independent experiments are shown (mean ± SE; n ≈ 100 stomata; **P < 0.01, Student’s t-test). Bar = 10 µm. b The mci1 mutant is insensitive to HCO3−/CO2 treatment. (Left) Images of wild-type and mci1 epidermal strips were taken, and guard cell images before and 60 min after addition of 3 mM KHCO3 are shown. (Right) Changes to the apertures (width/length) of stomatal pores in wild type and mci1 in response to 3 mM KHCO3. Data from three independent experiments are shown (mean ± SE; n ≈ 100 stomata; **P < 0.01, Student’s t-test). Bar = 10 µm. cmcs1 (left) and mci1 (right) exhibited abnormal AEQ luminescence intensities changes in response to 1 mM KHCO3. Leaves were put individually into the wells of a 96-well plate, and luminescence values were recorded at intervals of 0.2 s after 1 mM KHCO3 was added. Data for 59 leaves are shown (mean ± SE). Orange lines indicate mutants; blue lines indicate wild type (AQ). RLU, relative luminescence units