Fig. 2From: Arabidopsis guard cell CO2/HCO3− response mutant screening by an aequorin-based calcium imaging systemKHCO3 can induce stomatal closure in Arabidopsis thaliana whether in light or darkness. a KHCO3 induces stomatal closure under light. (Left) Guard cell images 20, 40, and 60 min after addition of 1 mM KHCO3. (Right) Changes in the apertures (width/length) of stomatal pores in response to 1 mM KHCO3. Data from three independent experiments are shown (mean ± SE; n ≈ 100 stomata; **P < 0.01, Student’s t-test). Bar = 10 µm. b KHCO3 induces stomatal closure under darkness. (Left) Guard cell images 5, 20, and 60 min after addition of 1 mM KHCO3. (Right) changes in the apertures (width/length) of stomatal pores in response to 1 mM KHCO3. Data from three independent experiments are shown (mean ± SE; n ≈ 100 stomata; **P < 0.01, Student’s t-test). Bar = 10 µmBack to article page