Fig. 3

Standard dilution series of plasmid standards. 10 point tenfold plasmid standard dilution series with a only 28S plasmid standard (singleplex) or b a combination of three different plasmid standards (multiplex) run in triplicates using a TaqMan^® assay and standard curves of the 28S plasmid and plasmid standards with phytoplasmal target including performance parameters of c singleplex and d multiplex assay The linear dynamic range is depicted as interpolation line with 95% confidence interval (dotted line) and calculated by a 4-point logarithmic nonlinear regression analysis. Error bars represent the SEM of the three technical triplicates