Fig. 2
From: Development of a universal endogenous qPCR control for eukaryotic DNA samples
Amplification with UNI28S primers in a SYBR-Green assay. Amplification of 28S using the UNI28 primers in a SYBR-Green assay. A serial dilution of pJET1.2-28S was prepared covering tenfold dilutions from 6.38 × 106 to 6.38 × 103 copies per reaction. Each dilution step was run as a triplicate. Red lines depict the samples containing the plasmid template in different dilutions and the green line represents the no template control (NTC; nuclease free water instead of template)