Fig. 1
From: Agrobacterium tumefaciens mediated transformation of the aquatic carnivorous plant Utricularia gibba
Protocol and transformation efficiency. a Diagram illustrating the protocol used for U. gibba transformation. U. gibba tissue, propagated for 1 month in liquid media, was collected and placed on absorbent paper to remove excess liquid. Collected tissue was grown for an additional week in fresh MS media containing BAP and then cut into small pieces with a scalpel. The segments of U. gibba tissue were transferred to MS with BAP and inoculated with an Agrobacterium culture overnight for co-cultivation. After 3 days of co-culture in continuous agitation, Agrobacterium was removed by filtering U. gibba tissue through a plastic mesh, followed by sterile water washes. Clean U. gibba tissue was placed in selective medium containing an antibiotic to kill remaining bacterial cells. b Box plot showing the number of transformants per gram of fresh weight carrying the p35S-GUS::GFP construct that were obtained when tissue was exposed to different concentrations of BAP. The results represent the means and standard deviations of four independent experiments