Table 1 Features calculated for each sorted particle using measurements by the large particle flow cytometer

Extinction

Integral optical density of the particle

Size

Calculated from Time-of-flight (TOF) using a calibration curve constructed with standard beads (size = 0.18 TOF + 38.32)

rF510

Fluorescence emission in the waveband 497–523 nm relative to average fluorescence emission across all wavebands

rF₅₄₃

Fluorescence emission in the waveband 531–555 nm relative to average fluorescence emission across all wavebands

rF₆₁₅

Fluorescence emission in the waveband 602–628 nm relative to average fluorescence emission across all wavebands

P

Maximum optical density in the optical density profile (Fig. 2)

P_x

Location within the optical density profile where the maximum occurs (Fig. 2)

C

Circularity index calculated from the optical density profile (Fig. 2)