Fig. 1
From: A small-scale fractionation pipeline for rapid analysis of seed mucilage characteristics
Schematic representation of pipeline used to extract small quantities of seed mucilage in discrete fractions for yield and chromatographic analysis. a Seeds and water are added to a microcentrifuge tube and incubated in a thermomixer-type shaking incubator at 25 °C for 1.5 h with agitation. b After centrifugation, the cold water extractable (CWE) mucilage is removed and water is added to the pellet to restore the working volume. c The sample is incubated at 65 °C for 1.5 h with agitation and after centrifugation the hot water extractable (HWE) mucilage is removed. Water is added to the pellet to restore the working volume. d Intense agitation by a mixer mill-type tissue disrupter is used to disrupt the seed-mucilage attachment. e After centrifugation, the intense extraction extractable (IAE) mucilage is removed. The pellet contains the demucilaged seeds (DMS). f All fractions can be freeze-dried for yield and chromatographic analysis