Table 1 Primers designed for quantitative real-time PCR
Primer names | Reference gene | GenBank Accession Number | Primer sequence (5′-3′) forward/reverse | Product Tm (°C) | Amplicon size (bp) | GC (%) | qRT-PCR efficiency (%) |
|---|---|---|---|---|---|---|---|
S37/S38 | Cs18S rDNA1 | AY563528.1 | GACTCCGCTGGCACCTTAT/GCCCTTCCGTCAATTCCT | 83.7 | 167 | 48 | 97.6–98.1 |
S572/S573 | GAPDH | KJ954782 | CCCGCATCTGGTAGACAAGA/TGATAGCATGTGTCCCTCCG | 81.0 | 82 | 50 | 92.0–98.8 |
S576/S577 | ITS | KJ955081 | AAAGGTAGTGGCGGACCCT/CCCAGTGCGAGACGTAAAGT | 82.5 | 62 | 58 | 96.0–97.6 |