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Fig. 1 | Plant Methods

Fig. 1

From: Development of a DNA-based real-time PCR assay for the quantification of Colletotrichum camelliae growth in tea (Camellia sinensis)

Fig. 1

Validation of primers for biomass quantification of tea plant and pathogen. a Amplification results for three genes using C. camelliae (C.c)-infected tea plant DNA. DNA. M: DL2000 Marker, 1: Cs18SrDNA1, 2: GAPDH, 3: ITS. bd Melting curve analyses of three genes. Each qRT-PCR product had a single melt curve indicating the breakdown of only one PCR product. b Cs18SrDNA1, c GAPDH, d ITS. eg The primer efficiency for the PCR quantification of the gene was determined using a serial dilution of DNA templates from tea plant, C. camelliae (C.c), and C. camelliae-infected tea plant (tea + C.c). e Cs18SrDNA1, f GAPDH, g ITS. The respective correlation coefficients (R2) are indicated

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