Fig. 4

Silencing of ChlI induced with the Cotton leaf crumple virus a Plants were infiltrated with the A. tumefaciens strain AGL1 carrying pCotton-ChlI or an empty-vector control. Phenotypes were visible approximately four weeks after infiltration with pCotton-ChlI (right). No phenotype was visible in control plants (left). b Overview on the efficiency of VIGS if plants were infiltrated with the A. tumefaciens strain AGL1. c Expression level of ChlI in plants showing a phenotype and empty-vector control plants were quantified with qPCR and normalized relative to the expression levels of eIFa and UBQ5. Error bars indicate standard error of five biological replicates. d Quantification of pigment content (chlorophyll a, chlorophyll b and carotenoids) of leaves from empty-vector control plants and ChlI-silenced plants. Error bars indicate standard error of 3 representative replicates