|0.15| °C in defined time windows, and that RH affected the shape of these thermal fingerprints. We demonstrated that IRT can also be used to assess the viability of the lichens Lobaria pulmonaria, Pseudevernia furfuracea and Peltigera leucophlebia. No clear relationship between aerobic metabolism and the shape of thermal fingerprints was found. Conclusions Infrared thermography appears to be a promising method for the diagnosis of viability of desiccation-tolerant tissues at early stages of water uptake. For seeds, it is possible to diagnose viability within the first hours of rehydration, after which time they can still be re-dried and stored until further use. We envisage our work as a baseline study for the use of IR imaging techniques to investigate physiological heterogeneity of desiccation tolerant life forms such as lichens, which can be used for biomonitoring, and for sorting live and dead seeds, which is potentially useful for the seed trade."/>
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Fig. 6 | Plant Methods

Fig. 6

From: Non-invasive diagnosis of viability in seeds and lichens by infrared thermography under controlled environmental conditions

Fig. 6

Thermal fingerprints of low- and high-molecular-weight carbohydrates upon hydration. a Thermal profiles of starch and glucose, both of which occur in seeds and lichens symbionts. b Thermal profiles of mannitol and ribitol, which frequently occur in lichens with green algal photobionts. Open blue horizontal bars indicate the time periods of hydration by water vapour and closed blue bars indicate imbibition with liquid water from below (as in Figs. 4, 5). Data are medians of n = 8 replicates

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