Fig. 5From: An efficient system composed of maize protoplast transfection and HPLC–MS for studying the biosynthesis and regulation of maize benzoxazinoidsAnalysis of the functions of ZmbHLH20 and ZmbHLH76 in regulating benzoxazinoids in maize protoplasts. A The change of benzoxazinoid contents induced by overexpressing and silencing ZmbHLH20 and ZmbHLH76 in maize protoplasts. B Transcript levels of BX1, BX2, BX3, BX4, BX5, BX6, BX7, BX8, BX9, and BX10/11 in maize protoplasts after overexpression or silencing of ZmbHLH20 and ZmbHLH76. Protoplasts transformed with empty vector were used as controls. Data are means ± SE. The letters a, b and c indicate significant differences among control, overexpression and gene-silenced protoplasts (ANOVA analysis; n = 4; *P ≤ 0.05)Back to article page