Fig. 2
From: High-throughput long paired-end sequencing of a Fosmid library by PacBio
The maps of the vectors pcc2FOS and pHZAUFOS3.A is the map of pcc2FOS. NotI was used to release the insert and the lacZ fragment was outside of CmR and oriV. B is the map of pHZAUFOS3. The LacZ fragment was moved between CmR and oriV; the two I-SceI sites adjacent to LacZ were used to release the insert, and another two I-SceI sites were used to break the vector skeleton into small fragments (2–3 kb)