Table 1 Composition of various media used in tissue culture and transformation of enset

Bud induction medium 1 (BIM1)

MS^a medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose and 2.5 mg/l BAP, pH5.8 and 3 g/l gelrite

Bud induction medium 2 (BIM2)

MS medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose and 5 mg/l BAP, pH5.8 and 3 g/l gelrite

Bud induction medium 3 (BIM3)

MS medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose and 10 mg/l BAP, pH5.8 and 3 g/l gelrite

Bud induction medium 4 (BIM4)

MS medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose and 15 mg/l BAP, pH5.8 and 3 g/l gelrite

Bud Induction Medium 5 (BIM5)

MS medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose and 22 mg/l BAP, pH5.8 and 3 g/l gelrite

Bud multiplication medium 1 (BMM1)

MS medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose, 0.175 mg/l IAA, 2.5 mg/l BAP, pH5.8 and 3 g/l gelrite

Bud multiplication medium 2 (BMM2)

MS medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose, 0.175 mg/l IAA, 5 mg/l BAP, pH5.8 and 3 g/l gelrite

Bud multiplication medium 3 (BMM3)

MS medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose, 0.175 mg/l IAA, 10 mg/l BAP, pH5.8 and 3 g/l gelrite

Bud multiplication medium 4 (BMM4)

MS medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose, 0.175 mg/l IAA, 15 mg/l BAP, pH5.8 and 3 g/l gelrite

Bud multiplication medium 5 (BMM5)

MS medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose, 0.175 mg/l IAA, 22 mg/l BAP, pH5.8 and 3 g/l gelrite

Shoot elongation medium 1 (SEM1)

MS medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose, 0.2% AC, 0.1 mg/l BAP, pH5.8 and 3 g/l gelrite

Shoot elongation medium 2 (SEM2)

MS medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose, 0.2% AC, 0.5 mg/l BAP, pH5.8 and 3 g/l gelrite

Shoot elongation medium 3 (SEM3)

MS medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose, 0.2% AC, 1.0 mg/l BAP, pH5.8 and 3 g/l gelrite

Shoot elongation medium 4 (SEM4)

MS medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose, 0.2% AC, 2.0 mg/l BAP, pH5.8 and 3 g/l gelrite

Shoot elongation medium 5 (SEM5)

MS^a medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose, 0.2% AC, 3.0 mg/l BAP, pH5.8 and 3 g/l gelrite

Root induction medium 1 (RIM1)

MS medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose, 1 mg/l IBA, pH5.8 and 3 g/l gelrite

Root induction medium 2 (RIM2)

MS medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose, 1 mg/l IBA, 0.1 mg/l BAP, pH5.8 and 3 g/l gelrite

Root induction medium 3 (RIM3)

MS medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose, 1 m g/l IBA, 0.25 mg/l BAP, pH5.8 and 3 g/l gelrite

Root induction medium 4 (RIM4)

MS medium supplemented with 10 mg/l ascorbic acid, 30 g/l sucrose, 1 m g/l IBA, 0.5 mg/l BAP, pH5.8 and 3 g/l gelrite

Cocultivation medium A (CCA)

MS medium supplemented with 30 g/l sucrose, 10 mg/l Ascorbic Acid, 1 mg/l BAP, pH 5.8 and 0.3% gelrite. Acetosyringone (200 µM) was added post-autoclaving

Cocultivation medium B (CCB)

MS medium supplemented with 30 g/l sucrose, 10 mg/l Ascorbic Acid, 1 mg/l BAP, 10 g/l glucose, pH 5.8 and 0.3% gelrite. Acetosyringone (200 µM) was added post-autoclaving

Regeneration medium (RM)

MS medium supplemented with 10 mg/l ascorbic acid, 1 mg/l BAP, 30 g/l sucrose, pH5.8 and 3 g/l gelrite

Infection medium (IM)

MS salts (M0222) supplemented with 10 mg/l ascorbic acid, 1 mg/l BAP, 30 g/l sucrose, pH 5.8, and 3 g/l gelrite and 200 µM acetosyringone added after autoclaving

Resting medium (RestM)

MS salts (M0222) supplemented with 10 mg/l ascorbic acid, 1 mg/l BAP, 30 g/l sucrose, pH 5.8, and 3 g/l gelrite and 300 mg/l cefotaxim added after autoclaving

Selective regeneration medium (SRM)

MS salts (M0222) supplemented with 10 mg/l ascorbic acid, 1 mg/l BAP, 30 g/l sucrose, pH 5.8, and 3 g/l gelrite and 300 mg/l cefotaxim and 150 mg/l kanamycin added after autoclaving