Fig. 3

Analysis of IAA metabolites synthesized by recombinant AtDAO1, AtGH3.6, AtGH3.17, AtUGT84B1 and AtUGT74D1 in bacterial assay. a oxIAA concentrations in AtDAO1-producing bacterial cultures. b, c IAAsp and IAGlu concentrations in AtGH3.6- (b) or AtGH3.17- (c) producing bacterial cultures. d, e IAGlc levels measured in AtUGT84B1- (d) or AtUGT74D1- (e) producing bacterial cultures. f Levels of IAA metabolites in control GFP-expressing bacterial cultures. All bacterial cultures were incubated with 0.1 mM IAA and with the corresponding cofactor mixture for 6 h at 20 °C and only supernatant fractions were analyzed. Bacterial cultures without IAA and cofactor mixture were used as mock samples. Mean ± SD (n = 3). N.D. not detectable