Fig. 2

Bacterial assay of IAA oxidation mediated by recombinant AtDAO1. a oxIAA concentrations in supernatant or pellet fractions from AtDAO1- or GFP-producing bacterial cultures supplemented with 0.001 mM IAA and with or without the DAO cofactor mixture. T0: samples taken before supplementation with exogenous IAA. b oxIAA concentrations in supernatant fractions from AtDAO1- or GFP-producing bacterial cultures supplemented with 0.01 mM IAA and with or without the DAO cofactor mixture for 1 or 6 h and incubated at either 20 °C or 30 °C. Bacterial cultures without IAA and the DAO cofactor mixture were used as mock samples. ‘No bacteria’, are media in which bacteria were not cultured. Mean ± SD (n = 3)