Fig. 2

Cryopreservation of Lilium pollen (a, b), seeds (c, d) and shoot tips (e–l). Oriental hybrid ‘Siberia’ pollen released from anthers and used for cryopreservation (a) and germinating pollen (b) after cryoexposure (courtesy of Prof. Yan Liu for providing the photos). Surface-disinfected seeds of Lilium brownie used for cryopreservation (c) and germinating seeds (d) after dehydration cryopreservation (courtesy of Dr. Liang Lin for providing the photos). Four-weeks old adventitious buds regenerated from leaf segments of Oriental hybrid ‘Siberia’ (e). A shoot tip excised from the 4-weeks old adventitious buds in d (f). PVS2 droplets, each containing one shoot tip, on an aluminum foil strip (g). Somatic embryo-like structures regenerated from cryopreserved shoot tips of Oriental hybrid ‘Siberia’ after 4 weeks of post-thaw culture (h). A germinating somatic embryo developed from h after 4 weeks of culture on germinating medium (i). A small leaf square-bearing two adventitious buds of Oriental hybrid ‘Siberia’ after 4 weeks of induction (j). Shoot regrowth of cryopreserved small leaf square-bearing adventitious buds after 4 weeks of post-thaw culture (k). A plantlet regenerated from cryopreserved shoot tips of Oriental hybrid ‘Siberia’ after 4 weeks of culture on rooting medium (l). Bars in a and b = 20 µm; in c and d = 2 mm; in e–l = 1 mm