Fig. 7From: Protocol for rapid clearing and staining of fixed Arabidopsis ovules for improved imaging by confocal laser scanning microscopy3D digital models with cellular resolution of wild-type Arabidopsis ovules. The models were obtained following deep-imaging of entire individual ovules. a Surface view. Left panel shows a stage 2-I ovule. Right panel depicts a stage 3 ovule. b Mid-sagittal sections through the 3D digital models depicted in a. c Number of cells per 3D digital ovule at three different stages during primordium formation. Mean ± SD is indicated. n = 5 for each stage. ap antipodal cells, cc central cell, es embryo sac, fu funiculus, ii inner integument, mmc megaspore mother cell, oi outer integument, pl placenta. Scale bars: 20 μmBack to article page