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Fig. 5 | Plant Methods

Fig. 5

From: Protocol for rapid clearing and staining of fixed Arabidopsis ovules for improved imaging by confocal laser scanning microscopy

Fig. 5

Compatibility with different fluorescent proteins. Mid-sagittal optical sections are shown. a–d Stage 2-II ovule from a Col-0 plant carrying the pPIN1::PIN1:GFP reporter [75]. a SR2200 stain. b Note the cell outlines marked by PIN1:GFP. c TO-PRO-3 iodide signal. d Merged channels. e–h Stage 3-V ovule from a Col-0 plant carrying a translational fusion of histone H2B to the fluorescent protein tdTomato under the control of the promoter of the ribosomal protein gene P16 (At3g60245) (pP16::H2B:tdTomato) [76]. Similar arrangement of images as depicted in a–d. f Note the nuclear H2B:tdTomato signal. i–l Stage 2-V ovule from a Col-0 plant carrying a pP16::PCNA2:mCitrine reporter (PCNA2: At2g29570). Similar arrangement of images as depicted in a–d. j Note the nuclear PCNA2:mCitrine signal. Scale bars: 20 μm

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Plant Methods

ISSN: 1746-4811