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TableĀ 1 Setup for measuring luciferase activity as proxy of cell death mediated by AVRa1 through recognition by Mla1 and AvrSr50 by recognition through Sr50

From: A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors

Sample 1 2 3 4 5 6 7 8 9 10 11 12
Reporter construct LUC LUC LUC LUC LUC LUC LUC LUC LUC LUC LUC LUC
NLR construct Mla1 Mla1 Mla1 Mla1 Mla1 Mla1 Sr50 Sr50 Sr50 Sr50 Sr50 Sr50
EV or AVR construct EV AVR a1 AVRa1-V1 AvrSr50 AvrSr50 RKQQC AvrSr50 QCMJC EV AVR a1 AVRa1-V1 AvrSr50 AvrSr50 RKQQC AvrSr50 QCMJC