Table 1 Setup for measuring luciferase activity as proxy of cell death mediated by AVRa1 through recognition by Mla1 and AvrSr50 by recognition through Sr50
Sample | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Reporter construct | LUC | LUC | LUC | LUC | LUC | LUC | LUC | LUC | LUC | LUC | LUC | LUC |
NLR construct | Mla1 | Mla1 | Mla1 | Mla1 | Mla1 | Mla1 | Sr50 | Sr50 | Sr50 | Sr50 | Sr50 | Sr50 |
EV or AVR construct | EV | AVR a1 | AVRa1-V1 | AvrSr50 | AvrSr50 RKQQC | AvrSr50 QCMJC | EV | AVR a1 | AVRa1-V1 | AvrSr50 | AvrSr50 RKQQC | AvrSr50 QCMJC |