Skip to main content
Fig. 7 | Plant Methods

Fig. 7

From: A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors

Fig. 7

Overview of steps for the adjustment of protoplast density and preparation of plasmid for transfection. Step 12: 0.5 ml of Wash Buffer containing protoplasts is transferred to cuvette. Step 13: Round bottom tube containing isolated protoplasts in Wash Buffer is placed in dark environment to let protoplasts settle for 45 min. Step 14: Protoplast concentration is determined. Step 15: Preparation of plasmid transfection mixtures. Step 16: Wash Buffer is removed from protoplast pellet using a pipette. Step 17: Transfection Buffer 1 is added to protoplast pellet for a calculated final OD600 = 0.4

Back to article page

Plant Methods

ISSN: 1746-4811