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Fig. 10 | Plant Methods

Fig. 10

From: A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors

Fig. 10

Overview of steps for protoplast recovery. Step 30: Up to six transfection samples tubes are centrifuged together at 1000 x g for 3 min. Step 31: Using a pipette, 965 µl of supernatant are remove from all transfection sample tubes. Step 32: 100 µl of 2× Cell Culture Lysis Buffer is transferred into each transfection sample tube. Step 33: Steps 30 to 32 are repeated for other transfection sample tubes

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Plant Methods

ISSN: 1746-4811