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Fig. 1 | Plant Methods

Fig. 1

From: A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors

Fig. 1

Schematic overview of experimental reasoning and expected outcomes. Viable wheat or barley protoplasts are transfected with plasmid mixtures and luciferase activity is determined as proxy for cell viability upon protein expression directed by transfected gene constructs. Reference sample contains constructs for LUC, NLR and EV to quantify reference luciferase activity when the NLR alone is overexpressed. In comparison to the reference samples, luciferase activity is expected to decrease only upon expression of matching NLR and AVR proteins (here NLR1 and AVR1)

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Plant Methods

ISSN: 1746-4811