Skip to main content

Table 2 Estimated total editing frequency for TaABCC6, TansLTP9.4 and TaNFXL1

From: An optimised CRISPR/Cas9 protocol to create targeted mutations in homoeologous genes and an efficient genotyping protocol to identify edited events in wheat

SampleEditing frequencyaSampleEditing frequencySampleEditing frequencySampleEditing frequency
ABCC6-19.3nsLTP9.4-19.1NFXL1-18.4pcoNFXL1-1b10.2
ABCC6-26.6nsLTP9.4-21.9NFXL1-218.3pcoNFXL1-28.6
ABCC6-39.1nsLTP9.4-311.3NFXL1-342.2pcoNFXL1-315.0
ABCC6-413.0nsLTP9.4-411.9NFXL1-422.8pcoNFXL1-421.2
ABCC6-59.0nsLTP9.4-50.0cNFXL1-50.0cpcoNFXL1-520.7
  1. aEditing frequency (%) = (reads with modification/(mapped reads + reads with modification)) × 100
  2. bpcoCas9 was used instead of crCas9 for the samples pcoNFXL1-1 to 5
  3. cNo modification was detected in those samples