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Table 2 Estimated total editing frequency for TaABCC6, TansLTP9.4 and TaNFXL1

From: An optimised CRISPR/Cas9 protocol to create targeted mutations in homoeologous genes and an efficient genotyping protocol to identify edited events in wheat

Sample

Editing frequencya

Sample

Editing frequency

Sample

Editing frequency

Sample

Editing frequency

ABCC6-1

9.3

nsLTP9.4-1

9.1

NFXL1-1

8.4

pcoNFXL1-1b

10.2

ABCC6-2

6.6

nsLTP9.4-2

1.9

NFXL1-2

18.3

pcoNFXL1-2

8.6

ABCC6-3

9.1

nsLTP9.4-3

11.3

NFXL1-3

42.2

pcoNFXL1-3

15.0

ABCC6-4

13.0

nsLTP9.4-4

11.9

NFXL1-4

22.8

pcoNFXL1-4

21.2

ABCC6-5

9.0

nsLTP9.4-5

0.0c

NFXL1-5

0.0c

pcoNFXL1-5

20.7

  1. aEditing frequency (%) = (reads with modification/(mapped reads + reads with modification)) × 100
  2. bpcoCas9 was used instead of crCas9 for the samples pcoNFXL1-1 to 5
  3. cNo modification was detected in those samples
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Plant Methods

ISSN: 1746-4811