Improving plant transient expression through the rational design of synthetic 5′ and 3′ untranslated regions

Table 2 The properties of the 3′UTRs used in this study

3′UTR	UTR length (bp)	GC content (%)	CA motifs	AAUAAA motifs	UUUU motifs	Free energy (kcal/mol)^a	CPMV 3′UTR Y-loop	Deliberately designed structure
3S0	202	36	20	3	5	− 17.5	No	None
3S1	200	34	25	4	6	− 13.1	No	None
3S2	50	36	7	1	1	− 1	No	None
3S3	135	34	9	1	5	− 23.7	No	None
3S4	175	38	11	1	1	− 28.92	Wt	Synthetic CITE^b
3S5	179	39	14	1	1	− 34.13	Wt	None
3S6	168	38	10	1	0	− 30.52	Totally divergent	Synthetic CITE^b
3S7	177	42	9	1	0	− 35.07	Totally divergent	Synthetic CITE^b + BTE^c
3S8	168	38	10	1	0	− 31.32	Partially divergent	Synthetic CITE^‡
3CPMV	185	33	8	0	5	− 28.73	Wt	None (viral sequence)

The properties of the 3′UTRs used in this study. ^a Measured by the mFold secondary structure prediction server [24]; ^b CITE: 3′Cap-independent translation enhancer; ^c BTE: barley yellow dwarf virus-like translation element

ISSN: 1746-4811