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Fig. 6 | Plant Methods

Fig. 6

From: Improving plant transient expression through the rational design of synthetic 5′ and 3′ untranslated regions

Fig. 6

Western blots assaying expression tests of three different proteins. The pEAQ, pHRE, and pHREAC vectors were used to transiently express three different proteins: the p24 protein of HIV (top), the coat protein of NNV (middle) and the capsid protein of chikungunya virus (bottom). Soluble protein extracts from three biological replicates (Rep 1,2, and 3) were electrophoresed on the same SDS-PAGE gel along with extract from empty vector (EV)—infiltrated leaves, then transferred to nitrocellulose membrane for protein-specific western blotting. After chemiluminescent detection, each membrane was stained with Ponceau Red to reveal protein loading based on the large subunit of RuBisCO (shown below each blot)

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Plant Methods

ISSN: 1746-4811