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Fig. 1 | Plant Methods

Fig. 1

From: Improving plant transient expression through the rational design of synthetic 5′ and 3′ untranslated regions

Fig. 1

The Synth expression cassette. Diagram of the Synth expression cassette used in this study, with arrows indicating location and cut sites of the different Type IIS restriction sites. These allow easy replacement of the UTRs or cloning site with new UTRs and ORFs of interest in one-pot restriction/ligation reactions. The complete annotated sequence of Synth can be found in Additional file 1

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Plant Methods

ISSN: 1746-4811