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Fig. 5 | Plant Methods

Fig. 5

From: An in vitro protocol for rapidly assessing the effects of antimicrobial compounds on the unculturable bacterial plant pathogen, Candidatus Liberibacter asiaticus

Fig. 5

Testing of synthetic antimicrobial peptides on CLas. Summary data from assessment of three putative AMPs, 1 mM streptomycin and 0.1% Triton-X 100. All samples underwent a 4 h incubation and received PMAxx @ 25 μM with light @15 min. The results from three separate experiments are summarized here. Each experiment contained twelve replicate samples per treatment and control. Values (ΔΔCt) are shown as the average difference between the intact/disrupted cell ratio (ΔCt of CLas 16 s rDNA from PMAxx compared to no PMAxx) for the experimental treatment compared to the intact/disrupted cell ratio of the no peptide (water) control. * Results with statistically significant increase in ΔΔCt compared to no-peptide control (P < 0.05) by non-parametric comparison

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