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Table 1 Stock solutions for RM10 and RM1

From: Regeneration and transient gene expression in protoplasts of Draparnaldia (chlorophytes), an emerging model for comparative analyses with basal streptophytes

No. Component Stock solution Stock concentration Final concentration in RM10 Final concentration in RM1
1 d-Mannitol in GM 6 g/100 mL GM Dissolve, adjust pH to 6.6 with NaOH/HCl, autoclave. 0.33 M 0.33 M 0.33 M
2 CaCl2·2H2O 2.94 g/40 mL dH2O Dissolve and autoclave. 0.5 M 10 mM 1 mM