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Table 1 Stock solutions for RM10 and RM1

From: Regeneration and transient gene expression in protoplasts of Draparnaldia (chlorophytes), an emerging model for comparative analyses with basal streptophytes

No.

Component

Stock solution

Stock concentration

Final concentration in RM10

Final concentration in RM1

1

d-Mannitol in GM

6 g/100 mL GM

Dissolve, adjust pH to 6.6 with NaOH/HCl, autoclave.

0.33 M

0.33 M

0.33 M

2

CaCl2·2H2O

2.94 g/40 mL dH2O

Dissolve and autoclave.

0.5 M

10 mM

1 mM

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Plant Methods

ISSN: 1746-4811