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Fig. 8 | Plant Methods

Fig. 8

From: Regeneration and transient gene expression in protoplasts of Draparnaldia (chlorophytes), an emerging model for comparative analyses with basal streptophytes

Fig. 8

Expression and regeneration of electroporated protoplasts. a–c Expression in protoplasts. a YFP channel (488 nm). b Chlorophyll channel (561 nm). c Merged. Pictures were taken 3 days after electroporation. d–f Negative control. d YFP channel (488 nm). A weak background fluorescent from the chloroplast is visible. e Chlorophyll channel (561 nm). f Merged. Pictures were taken 3 days after electroporation. g Regenerated filaments from electroporated protoplasts. A picture taken 15 days after electroporation at 300 V. Scale bar: a–f = 5 μm; g = 500 μm

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Plant Methods

ISSN: 1746-4811