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Fig. 7 | Plant Methods

Fig. 7

From: Second generation of pepino mosaic virus vectors: improved stability in tomato and a wide range of reporter genes

Fig. 7

PepMV-induced subcellular bodies in N. benthamiana and tomato plants. a and b CLSM images of JLPepGFPm2 infection in N. benthamiana at 3 d post inoculation (dpi). b High magnification of a fluorescent body in JLPepGFPm2 infection to show its cytoplasmic localization and spatial relation with the nucleus. c and d CLSM images of PepDsRed infection in tomato at 3 dpi. d High magnification of a fluorescent body in PepDsRed infection. e Distribution of the endoplasmic reticulum marker (ER-mCherry) at 3 dpi in N. benthamiana in the absence of PepMV infection. In the presence of JLPepGFPm2 infection: f changes of ER-mCherry localization were observed, g green fluorescent bodies of JLPepGFPm2 and h merged image of f and g to see the matching. High magnification images: i the distribution of ER-mCherry during the infection, j the PepMV subcellular body and k merged image of i and j to see the incomplete matching between the red and the green labelling in the body. l Distribution of Golgi-mCherry marker at 3 dpi in N. benthamiana in the absence of PepMV infection. In the presence of JLPepGFPm2 infection: m changes of Golgi-mCherry localization were observed (n), green fluorescent bodies of JLPepGFPm2 and o merged image of (m) and (n) to see the matching. High magnification images of: p the distribution of Golgi-mCherry during the infection, q the PepMV subcellular body and r merged image of (p) and (q) to see the incomplete matching between the red and the green labelling in the body. N, nucleus. Blue colour corresponds to chloroplasts autofluorescence

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