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Table 1 Optimised DF method for circadian phenotyping of Brassica and wheat leaves

From: A high-throughput delayed fluorescence method reveals underlying differences in the control of circadian rhythms in Triticum aestivum and Brassica napus

Species

Plant age (days after sowing)

Leaf age (1 = oldest leaf)

Cut sample

Light regime

Temperature (°C)

Throughput (N/imaging cabinet)

% return (from period estimation algorithms)

Brassica

20

1

3 cm square

L:L

22

36

96.3

Wheat

25

2

10 cm section

D:D

27

44–48

98.8

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Plant Methods

ISSN: 1746-4811