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Fig. 6 | Plant Methods

Fig. 6

From: A high-throughput delayed fluorescence method reveals underlying differences in the control of circadian rhythms in Triticum aestivum and Brassica napus

Fig. 6

DF can be used to measure period differences between elite cultivars in Brassica and wheat. 10 cm sections from the second leaf of 25 day old wheat seedlings were imaged under D:D at 27 °C. 3 cm square sections from the first leaf of 21 day old Brassica seedlings were imaged under L:L at 22 °C. Period boxplots based on the DF oscillations from different cultivars are shown for wheat (a) and Brassica (b). The three replicates in the Brassica data represent different seed batches. Period values were estimated using FFT NLLS, BAMP dtr, 24–120 h cut-off window. BAMP de-trended DF data was normalised to the mean DF intensity across all cultivars and plotted against time in hours after dawn (c, d). Brassica data represents results from two cabinets normalised for between-cabinet effects. Wheat data represents results from two identical experiments with two imaging cabinets run in parallel as technical replicates and normalised for the between-cabinet and experimental-run effects. Wheat: Robigus (N = 25/25), Cadenza (N = 12/12), Chinese Spring (N = 24/25), Claire (N = 27/27), Weebil (N = 22/24), Paragon (N = 24/4), Norin-61 (N = 25/25). Brassica: for each seed batch of Cabriolet, Chuanyou II, Norin (N = 8/8). Brassica data is consistent with previous experiments shown in Additional file 1: Supplementary material S14. Significance codes: for wheat cultivars (a), ***p < 0.001, **p < 0.01 relative to either a = Norin 61 or b = Paragon. For brassica cultivars (b), significantly different periods (p < 0.05) are labelled relative to a = Norin Y1, b = Norin Y2 or c = Norin Y3

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