Fig. 1

DF rhythms in wheat change with the age of the plant and between leaves on the same plant. The wheat plant age experiment (a, b) used ‘leaf 2’ from plants grown for 18, 25, 32 or 39 days. Blue boxplots show differences in period (a) and RAE (b) at each plant age. The wheat leaf variation experiment used 4 leaves sampled from 25 day old plants following the leaf numbering system described in c. Orange boxplots show differences in period (d) and RAE (e) at each leaf age. Colour scales reflect an ageing gradient with lighter colours representing younger material. Data represents results from two imaging cabinets run in parallel as technical replicates and normalised for the between-cabinet effects. Period estimates were calculated using FFT-NLLS (BAMP de-trended data, 24–120 h cut-off). N values reflect the number of samples for which period was estimated out of the total number of individuals sampled. Age 18 (N = 26/26), age 25 (N = 24/24), age 32 (N = 25/26), age 39 (N = 19/23). Leaf 1 (N = 22/22), leaf 2 (N = 22/22), leaf 3 (N = 22/22), leaf 4 (N = 21/22). Significance codes: ***p < 0.001, **p < 0.01 *p < 0.05