Skip to main content
Fig. 1 | Plant Methods

Fig. 1

From: Accurate and efficient amino acid analysis for protein quantification using hydrophilic interaction chromatography coupled tandem mass spectrometry

Fig. 1

a Chromatographic separation of twenty amino acids using a Z-HILIC column b. Leucine and isoleucine were separated capitalizing on the HILIC-water layer. (i) Leucine and isoleucine are not resolved with a linear gradient 10–50% solvent A over eight minutes. (ii–iv) Represent increased separation of these compounds with changes in gradient over the first 2 min (ii 0–25% solvent A, iii 0–15% solvent A, iv 0–10% solvent A). *Cysteine was detected as cystine. Ile was quantified using a distinct mass trace (Additional file 1: supplementary Table 1)

Back to article page

Plant Methods

ISSN: 1746-4811