Fig. 1
From: Centromere histone H3- and phospholipase-mediated haploid induction in plants
An illustration of the methods to modify CENH3 for HI. The modifications at N-terminal tail of CENH3 include direct conjugation of GFP tag (GFP Fusion) and replacement of the CENH3 tail with L. oleraceum tail as well as H3.3 tail with or without a GFP tag (Tail Swap). The modifications at C-terminus are mainly generated by the point mutation in the HFD region (HFD Point Mutation)