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Table 2 Types and frequency of sequence mutation in exon 7 of the PDS gene in Fragaria

From: CRISPR/Cas9-mediated mutagenesis of phytoene desaturase in diploid and octoploid strawberry

Sequence variant

Hawaii 4

Calypso

Non-synonymous amino acid substitution

Frameshift and protein truncation

Amino acid deletion

Putative functional protein

− 1:1D

17

4

–

+

+

–

− 2:1D

2

–

–

+

+

–

− 2:2D

10

3

–

+

+

–

− 1:3D

11

3

–

–

+

–

− 4:4D

–

1

–

+

+

–

− 1:5D

2

3

–

+

+

–

− 2:6D

1

–

–

–

+

–

− 3:7D

–

1

–

+

+

–

− 5:7D

–

1

–

+

+

–

− 2:9D

1

–

–

–

+

–

− 5:9D

–

1

–

–

+

–

− 6:9D

1

–

–

–

+

–

− 10:9D

1

–

–

–

+

–

− 11:15D

5

–

–

–

+

–

− 1:22D

1

–

–

+

+

–

− 25:27D

2

–

–

–

+

–

− 44:45D

1

–

–

+

+

–

1:3D

2

–

–

–

+

–

1:5D

1

–

–

+

+

–

1:1I

3

–

+

+

–

–

SNV:− 3A and 1:2

1

–

+

+

–

–

SNV:1A

1

–

+

–

–

+

  1. The numbers of shoot lines with each variant type are shown for CRISPR/Cas9 transgenic lines of ‘Hawaii 4’ and ‘Calypso’. Each sequence variant type is identified by the location of the mutation relative to the cut site:number bases deleted (D), inserted (I), or substituted (SNV:location relative to cut site and base substitution). The resultant amino acid changes and consequences for PDS protein function for each sequence variant type are indicated by ‘+’ or ‘−’. Amino acid substitutions (generated by insertions or base substitutions) are non-synonymous