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Table 2 Types and frequency of sequence mutation in exon 7 of the PDS gene in Fragaria

From: CRISPR/Cas9-mediated mutagenesis of phytoene desaturase in diploid and octoploid strawberry

Sequence variant Hawaii 4 Calypso Non-synonymous amino acid substitution Frameshift and protein truncation Amino acid deletion Putative functional protein
− 1:1D 17 4 + +
− 2:1D 2 + +
− 2:2D 10 3 + +
− 1:3D 11 3 +
− 4:4D 1 + +
− 1:5D 2 3 + +
− 2:6D 1 +
− 3:7D 1 + +
− 5:7D 1 + +
− 2:9D 1 +
− 5:9D 1 +
− 6:9D 1 +
− 10:9D 1 +
− 11:15D 5 +
− 1:22D 1 + +
− 25:27D 2 +
− 44:45D 1 + +
1:3D 2 +
1:5D 1 + +
1:1I 3 + +
SNV:− 3A and 1:2 1 + +
SNV:1A 1 + +
  1. The numbers of shoot lines with each variant type are shown for CRISPR/Cas9 transgenic lines of ‘Hawaii 4’ and ‘Calypso’. Each sequence variant type is identified by the location of the mutation relative to the cut site:number bases deleted (D), inserted (I), or substituted (SNV:location relative to cut site and base substitution). The resultant amino acid changes and consequences for PDS protein function for each sequence variant type are indicated by ‘+’ or ‘−’. Amino acid substitutions (generated by insertions or base substitutions) are non-synonymous