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Fig. 1 | Plant Methods

Fig. 1

From: Quantitative assay of targeted proteome in tomato trichome glandular cells using a large-scale selected reaction monitoring strategy

Fig. 1

Schematic overview of the development of selected reaction monitoring (SRM) assays. All mass spectrometry (MS) analyses were conducted using a triple quadrupole/linear ion trap hybrid MS instrument connected with nanoflow reversed-phase liquid chromatography (RPLC). First, the glandular cell samples collected from the target trichome were subjected to a shotgun proteomics analysis. Based on proteomics information, suitable peptides for an SRM experiment were selected and SRM assays targeting selected peptides were designed. The established assay performance was assessed using the rest of the peptide samples used in the shotgun analysis

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Plant Methods

ISSN: 1746-4811