Fig. 3From: Selection and validation of reference genes for quantitative expression analysis of miRNAs and mRNAs in PoplarThe optimal number of reference genes for accurate normalization calculated by geNorm during plant regeneration. Pairwise variation (Vn/Vn+1) analysis of 18 candidate reference genes analyzed in four sample subsets. callus, callus and shoots induced by 84K leaves; seedling, various tissues from 84K seedling stage; plant, various tissues from 84K plant stage; ALL, all samples from callus, seedling and plant developmental stagesBack to article page