Evaluation of reference genes for normalizing RT-qPCR in leaves and suspension cells of Cephalotaxus hainanensis under various stimuli

Table 3 Ranking of 9 candidate reference genes according to BestKeeper

Rank	Leaf-ABA			Leaf-Ethylene			Leaf-Mannitol			Leaf-MeJA			Leaf-NaCl			Leaf-SA			Leaf-Total
Rank	Gene	SD	CV	Gene	SD	CV	Gene	SD	CV	Gene	SD	CV	Gene	SD	CV	Gene	SD	CV	Gene	SD	CV
1	18S	0.37	2.33	18S	0.50	3.15	18S	0.49	2.97	PP2C	0.59	2.38	18S	0.40	2.48	18S	0.62	3.94	18S	0.69	4.30
2	UBC	0.75	2.90	TUA	0.71	2.29	NAC	0.69	2.27	18S	0.73	4.53	TUB	0.53	2.00	UBQ	0.89	4.07	UBQ	0.98	4.40
3	PP2C	0.96	3.75	ACT	0.83	2.74	UBQ	0.71	3.22	UBQ	0.77	3.68	UBQ	0.54	2.37	NAC	0.97	3.29	PP2C	1.10	4.21
4	UBQ	0.96	4.30	UBQ	0.92	4.13	TUA	0.90	2.87	ACT	0.79	1.37	UBC	0.55	2.00	TUA	0.98	3.25	TUA	1.14	3.74
5	NAC	1.02	3.48	UBC	1.03	3.75	PP2C	0.91	3.52	NAC	0.97	3.49	PP2C	0.64	2.41	F-box	0.99	3.62	TUB	1.15	4.44
6	TUA	1.09	3.54	TUB	1.06	4.01	F-box	1.07	3.83	UBC	1.05	4.21	NAC	0.69	2.23	TUB	1.09	4.35	F-box	1.19	4.30
7	ACT	1.18	4.03	NAC	1.06	3.49	UBC	1.07	3.90	TUA	1.05	3.55	F-box	0.85	2.99	PP2C	1.10	4.25	NAC	1.25	4.24
8	TUB	1.24	4.94	PP2C	1.14	4.23	TUB	1.12	4.27	F-box	1.06	4.04	TUA	1.05	3.39	UBC	1.14	4.35	ACT	1.31	4.34
9	F-box	1.27	4.57	F-box	1.27	4.52	ACT	1.16	3.87	TUB	1.11	4.47	ACT	1.16	3.74	ACT	1.20	4.04	UBC	1.32	4.98

Rank	Cell-ABA			Cell-Ethylene			Cell-Mannitol			Cell-MeJA			Cell-NaCl			Cell-SA			Cell-Total
Rank	Gene	SD	CV	Gene	SD	CV	Gene	SD	CV	Gene	SD	CV	Gene	SD	CV	Gene	SD	CV	Gene	SD	CV
1	18S	0.22	1.79	UBC	0.46	2.33	18S	0.58	4.25	18S	0.42	3.28	18S	0.24	1.88	UBQ	0.27	1.68	18S	0.62	4.74
2	UBC	0.37	1.87	18S	0.46	3.57	UBQ	0.66	4.10	NAC	0.56	2.29	UBC	0.50	2.52	18S	0.31	2.23	UBC	0.82	4.06
3	TUA	0.47	2.18	UBQ	0.47	3.04	TUA	0.78	3.55	UBC	0.63	3.09	UBQ	0.61	4.07	UBC	0.52	2.48	TUA	0.94	4.28
4	TUB	0.54	2.67	TUA	0.66	3.04	TUB	0.97	4.61	TUA	1.08	4.94	TUA	0.62	2.88	NAC	0.59	2.48	TUB	1.02	4.87
5	UBQ	0.60	3.96	TUB	0.84	4.07	NAC	1.06	4.45	UBQ	1.08	5.85	TUB	0.63	3.09	TUB	0.78	3.66	UBQ	1.07	6.71
6	PP2C	0.69	3.41	NAC	0.90	3.98	UBC	1.17	5.66	TUB	1.28	6.01	ACT	0.90	3.95	TUA	0.95	4.21	NAC	1.22	5.31
7	NAC	1.04	4.68	PP2C	1.06	4.76	PP2C	1.27	5.59	ACT	1.70	7.02	PP2C	0.94	4.32	ACT	0.97	4.09	ACT	1.38	5.83
8	F-box	1.04	4.61	ACT	1.27	5.43	ACT	1.52	6.40	PP2C	2.33	8.89	NAC	0.95	4.22	PP2C	1.56	6.58	F-box	2.14	8.80
9	ACT	1.03	4.44	F-box	1.40	6.13	F-box	2.11	9.04	F-box	3.17	11.86	F-box	1.42	6.33	F-box	2.32	9.15	PP2C	2.14	9.29

ISSN: 1746-4811