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Table 2 Ranking of 9 candidate reference genes according to NormFinder

From: Evaluation of reference genes for normalizing RT-qPCR in leaves and suspension cells of Cephalotaxus hainanensis under various stimuli

Rank

Leaf-ABA

Leaf-Ethylene

Leaf-Mannitol

Leaf-MeJA

Leaf-NaCl

Leaf-SA

Leaf-Total

1

18S(0.316)

18S(0.152)

PP2C(0.209)

UBQ(0.249)

TUB(0.128)

18S(0.299)

18S(0.267)

2

PP2C(0.332)

F-box(0.262)

18S(0.238)

PP2C(0.259)

UBQ(0.199)

F-box(0.346)

UBQ(0.336)

3

UBQ(0.374)

UBQ(0.277)

UBQ(0.242)

18S(0.282)

UBC(0.207)

TUB(0.421)

PP2C(0.356)

4

TUB(0.409)

TUB(0.287)

F-box(0.251)

F-box(0.393)

18S(0.252)

UBC(0.444)

F-box(0.363)

5

ACT(0.570)

UBC(0.298)

TUB(0.369)

UBC(0.471)

PP2C(0.299)

PP2C(0.474)

TUB(0.392)

6

NAC(0.575)

TUA(0.354)

UBC(0.417)

TUB(0.621)

F-box(0.320)

UBQ(0.507)

UBC(0.440)

7

F-box(0.598)

PP2C(0.502)

NAC(0.455)

ACT(0.648)

TUA(0.462)

ACT(0.570)

NAC(0.454)

8

UBC(0.613)

ACT(0.504)

ACT(0.463)

NAC(0.687)

NAC(0.699)

TUA(0.586)

ACT(0.489)

9

TUA(0.686)

NAC(0.611)

TUA(0.579)

TUA(0.808)

ACT(0.927)

NAC(0.723)

TUA(0.497)

Rank

Cell-ABA

Cell-Ethylene

Cell-Mannitol

Cell-MeJA

Cell-NaCl

Cell-SA

Cell-Total

1

UBC(0.230)

TUA(0.103)

18S(0.277)

18S(0.578)

TUA(0.214)

18S(0.320)

18S(0.376)

2

TUA(0.285)

18S(0.218)

TUA(0.300)

ACT(0.609)

18S(0.246)

ACT(0.419)

UBC(0.380)

3

18S(0.317)

UBC(0.254)

UBC(0.517)

UBC(0.685)

UBC(0.333)

UBC(0.484)

TUA(0.436)

4

PP2C(0.344)

PP2C(0.392)

PP2C(0.539)

TUB(0.700)

PP2C(0.394)

UBQ(0.501)

ACT(0.530)

5

TUB(0.454)

TUB(0.416)

UBQ(0.652)

UBQ(0.727)

ACT(0.403)

TUB(0.526)

UBQ(0.575)

6

UBQ(0.526)

ACT(0.483)

TUB(0.665)

NAC(0.811)

TUB(0.502)

TUA(0.574)

TUB(0.669)

7

ACT(0.696)

UBQ(0.613)

ACT(0.679)

TUA(0.825)

UBQ(0.595)

PP2C(0.789)

PP2C(0.673)

8

NAC(0.744)

F-box(0.864)

NAC(0.964)

PP2C(1.328)

NAC(0.708)

NAC(0.844)

NAC(0.911)

9

F-box(0.755)

NAC(1.103)

F-box(1.167)

F-box(1.810)

F-box(0.861)

F-box(1.394)

F-box(0.952)