Fig. 2

Measurement of enhancer activity based on bioluminescent imaging in vivo. a A representative N. benthamiana leaf infiltrated with constructs containing six different enhancers (sample 2, 3 and 5–8), the 35S promoter (positive control, sample1) and a mini35S promoter (negative control, sample 4). Data was collected at 30 h after agroinfiltration. Color scale of the luminescent signal intensity; purple, least intense signal; red, most intense signal; Inner gray for sample 1, over saturated intense signal. b Three-dimension bioluminescent signal of (A). c Three-dimension bioluminescent signal of (A) after excluding sample 1