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Fig. 7 | Plant Methods

Fig. 7

From: Multicolour three dimensional structured illumination microscopy of immunolabeled plant microtubules and associated proteins

Fig. 7

Quantitative colocalization of MPK6 and pERK by means of CLSM and SIM. a Nuclear colocalization of MPK6 (a; left panel), pERK species (a; middle panel) and their overlay (a; right panel) after CLSM documentation. b Nuclear colocalization of MPK6 (b; left panel), pERK species (b; middle panel) and their overlay (b; right panel) after SIM documentation. Dashed white lines delineate the nucleus. c Fluorescence intensity correlation between the signal corresponding to MPK6 and the signal corresponding to pERK from (a) showing positive correlation with a Manders coefficient of 0.972. d Fluorescence intensity correlation between the signal corresponding to MPK6 and the signal corresponding to pERK from (b) showing absence of correlation with a Manders coefficient of 0. Bars = 2 μm

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Plant Methods

ISSN: 1746-4811